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| Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da. |
| ABSTRACT: The aim of the present study was to investigate the correlations among chronic inflammatory reaction, immunostaining and parasite load in the genital system of female dogs naturally infected with Leishmania infantum . Animals (n = 10) used in this study were from the Department of Vector Control and Animal Surveillance of the municipality of Caruaru, state of Pernambuco, Brazil. Fragments of the vulva, vagina, cervix, uterine body, uterine horns and ovaries were submitted to histopathological analysis, immunohistochemistry (IHC) and DNA detection of amastigotes by qPCR. Correlations were found between the IHC findings and chronic inflammatory infiltrate related to L. Infantum only in the vulva and vagina; whereas, the same inflammatory reactions... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine visceral leishmaniasis; Genital pathology; Immunohistochemistry; QPCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001102029 |
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| Oliveira,Vinícius Vasconcelos Gomes de; Macedo,Simone Regina Barros de; Araújo,Flábio Ribeiro de; Ramos,Carlos Alberto do Nascimento; Ramos,Rafael Antonio Nascimento; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da. |
| ABSTRACT: Canine visceral leishmaniasisis an important disease caused by the protozoon Leishmania infantum which affects several organs and systems, including the genital tract. The L. infantum tropism to the male genital system and correlation among parasite load, immunohistochemistry detection and structural changes in these organs is controversy. Therefore, the aim of this study was to evaluate this correlation in the genital organs of the male dogs naturally infected with L. infantum. Samples from testicles, epididymis, prostate, glans penis, prepuce and scrotum were collected from 19 positive adult dogs. Structural changes were observed in the testicles (5.2%), epididymis (2.6%), prepuce (5.2%) and scrotum (5.2%) of the samples positive at... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canine visceral leishmaniasis; Structural changes; Genital pathology; Immunohistochemistry. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000400641 |
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| Oliveira,Vinícius Vasconcelos Gomes de; Ramos,Rafael Antonio Nascimento; Ramos,Carlos Alberto do Nascimento; Guerra,Neurisvan Ramos; Maia,Frederico Celso Lyra; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da. |
| ABSTRACT: Visceral leishmaniasis (VL) is caused by the protozoon Leishmania infantum . Transmission of this parasite to hosts occurs mainly through the bite of infected sand flies. However, alternative infection routes have been hypothesized, especially in areas where the biological vector is absent. The exact time of infection and whether in utero transmission occurs have still not been fully elucidated. This report demonstrates molecular evidence of vertical transmission of L. infantum from a pregnant dog to the embryo. Samples (e.g. vulva, vagina, cervix, uterine body, uterine horn and ovaries) from a female naturally infected by L. infantum and from her embryo were molecularly analyzed by means of qPCR and cPCR followed by DNA sequencing. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmaniasis; Dog; Vertical infection; Embryo. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000100553 |
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| Oliveira,Vinícius Vasconcelos Gomes de; Alves,Leucio Câmara; SilvaJunior,Valdemiro Amaro da. |
| A leishmaniose visceral canina (LVC) é uma doença parasitária crônica causada por protozoários pertencentes ao gênero Leishmania. No Brasil, a transmissão se dá, principalmente, pela ação hematófaga de insetos vetores pertencentes à subfamília Phlebotominae, particularmente, a espécie Lutzomyia longipalpis. Todavia, a trasmissão vertical e venérea da LVC está presente. Os principais sinais clínicos observados nos animais acometidos pela LVC são linfoadenomegalia, dermatopatias, hepatoesplenomegalia, onicogrifose e oftalmopatias, contudo quadros atípicos podem ser observados, inclusive com o envolvimento do sistema genital. Dessa forma, o objetivo deste artigo é realizar revisão sobre as principais patologias genitais em cães machos e fêmeas com... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania sp; Cães; Alterações etruturais; Sistema genital. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000900016 |
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| Oliveira,Vinícius Vasconcelos Gomes de; Alves,Leucio Câmara; Silva Junior,Valdemiro Amaro da. |
| Visceral leishmaniasis (VL) is a chronic disease caused by Leishmania infantum. The major sites of parasite localization in infected animals are the secondary lymphoid organs, bone marrow and cutaneous tissue. However, reports exist on the detection of the parasite in the organs of the male and female genital system. The main route of transmission is related to the hematophagous sandfly vectors of the genus Lutzomyia(New World) and Phlebotomus(Old World). However, other routes of transmission may be mentioned, such as sexual, vertical, hematogenic without vector and others involved in VL epidemiology. Thus, the current article reviews the main forms of transmission of visceral leishmaniasis in mammals |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania sp; Dogs; Sexual transmission; Vertical transmission. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000901622 |
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| Brito,Fábio Luiz da Cunha; Alves,Leucio Câmara; Duque Ortiz,Juan Pablo; Maia,Federico Celso Lyra; Silva Junior,Valdemiro Amaro da; Laus,Jose Luiz. |
| Among the parasitic diseases, Canine Visceral Leishmaniasis (CVL) is included in the systemic illnesses of chronic evolution that attack men and dogs, presenting varied clinical manifestations as cachexia, dermatologic lesions, peripheral lymphadenopathies, besides the ocular lesions. This work report the case of a dog clinically suspected of having CVL, presenting skin lesions, cachexia, gryphosis, and ocular signs of uveitis. The parasitological diagnosis was accomplished for Canine Leishmaniasis through the visualization of amastigote forms of Leishmania chagasi in smears of bone marrow fluid aspirate, of non-lesioned, and lesioned skin. Alterations in the ocular structures are characterized mainly by mononuclear-plasmocitic infiltrate. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Uveitis; Leishmania sp; Dog. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000300042 |
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| Carvalho,Gílcia Aparecida de; Alves,Leucio Câmara; Maia,Rafael Trindade; Andrade,Carlos Fernando Salgueirosa de; Ramos,Rafael Antonio do Nascimento; Faustino,Maria Aparecida da Glória. |
| The metropolitan region of Recife, Brazil is endemic for Dirofilaria immitis and has an environment favorable to the development of Culex quinquefasciatus. The goal of this study was to evaluate the vector competence of the Cx. quinquefasciatus RECIFE population for D. immitis transmission. A total of 2,104 females of Cx. quinquefasciatus RECIFE population were exposed to different densities of D. immitis microfilariae blood meals, ranging from 1,820 to 2,900 mf/ml of blood, in a natural membrane apparatus. The results showed a variation between 92.3% and 98.8% of females fed. The exposure of the Cx. quinquefasciatus RECIFE population to different densities of microfilariae did not influence the mortality of the mosquitoes. Infective larvae from D. immitis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biology; Experimental infections; Mosquitoes. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262008000400018 |
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